{"id":88,"date":"2018-03-07T13:53:36","date_gmt":"2018-03-07T12:53:36","guid":{"rendered":"http:\/\/malcolmbuckle.com\/?p=88"},"modified":"2022-09-28T12:07:12","modified_gmt":"2022-09-28T10:07:12","slug":"phast-technology","status":"publish","type":"post","link":"http:\/\/malcolmbuckle.com\/index.php\/2018\/03\/07\/phast-technology\/","title":{"rendered":"PhAST Technology"},"content":{"rendered":"

Over a long period I have developed \u00a0an \u00a0approach using the probability of UV induced cyclobutane dimer formation between adjacent pyrimidines on the same DNA chain \u00a0to follow changes in local base structure in DNA and nucleoprotein complexes. This technique called Photo-chemical Analysis of Structural Transitions (PhAST) promotes DNA pyrimidine dimer formation. The location and intensities of this photo-chemistry are affected by external agents that reshape the DNA structure. Thus, the technique provides unique information on local structure at the DNA nucleotide level. We reveal photochemical products using a simple primer extension technique coupled to capillary electrophoresis (now being extended to NextGen sequencing!). Our approach confers high-resolution, excellent quantification, application in vitro <\/em>and in vivo<\/em>, and the possibility of high-throughput since practically any size DNA sample may be analysed.<\/p>\n

Recent results on nucleosomes include\u00a0\u00a0H<\/span>atakeyama 2016<\/a>\u00a0and\u00a0Hatakeyama 2020<\/a><\/strong>\u00a0and\u00a0<\/span>demonstrated that, for the first time, we are able to follow dynamic changes at a molecular level in DNA during nucleosome assembly and disassembly.<\/span><\/p>\n

The technique has also been applied to liquid crystal DNA : \u00a0Brach <\/strong><\/a>2018<\/a><\/strong>.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Over a long period I have developed \u00a0an \u00a0approach using the probability of UV induced cyclobutane dimer formation between adjacent pyrimidines on the same DNA chain \u00a0to follow changes in local base structure in DNA and nucleoprotein complexes. This technique called Photo-chemical Analysis of Structural Transitions (PhAST) promotes DNA pyrimidine dimer formation. The location and … <\/p>\n

Continue reading “PhAST Technology”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[7],"tags":[8,9],"class_list":["post-88","post","type-post","status-publish","format-standard","hentry","category-science","tag-phast","tag-publications"],"yoast_head":"\nPhAST Technology - Malcolm Buckle<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/malcolmbuckle.com\/index.php\/2018\/03\/07\/phast-technology\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PhAST Technology - Malcolm Buckle\" \/>\n<meta property=\"og:description\" content=\"Over a long period I have developed \u00a0an \u00a0approach using the probability of UV induced cyclobutane dimer formation between adjacent pyrimidines on the same DNA chain \u00a0to follow changes in local base structure in DNA and nucleoprotein complexes. This technique called Photo-chemical Analysis of Structural Transitions (PhAST) promotes DNA pyrimidine dimer formation. 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