Malcolm Buckle is currently a Director of Research in the CNRS in France and from 2011 to 2020 was director of a fundamental biology research laboratory (LBPA) at the Ecole Normale Supérieure de Paris-Saclay in Cachan France.
Following a PhD in Solid Phase Peptide Synthesis with Roger Epton at Wolverhampton Polytechnic in the UK, he held a series of post-doctoral positions in Canada, Italy and France and finally became a senior scientist at the CNRS working in Pr Henri Buc’s lab at the Pasteur Institute Paris.
After moving to the ENS Cachan, from 2002 to 2015 he directed a group working on the control and regulation of transcription and although his research interests have covered a fairly broad spectrum ranging from solid phase peptide synthesis through bioenergetics to the control of gene expression, he has always been struck by the necessity of developing techniques that allow time-resolved analysis of dynamic systems. This has led to the development and use of rapid high-energy UV lasers to follow the photo-reactivity of DNA involved in nucleoprotein complexes. The application of this technique (called PhAST after Photochemical Analysis of Structural Transitions) to nucleosomes in vitro has completely changed our way of thinking about nucleosome positioning.
Malcolm was an early user of emerging technologies based on Surface Plasmon Resonance (SPRi) and realised early that these techniques were severely limited by problems associated with the interface between a surface-based biosensor surface (a biochip) and macromolecules in solution. The team of Dynamics of Macromolecular Complexes at the LBPA developed a unique surface chemistry with a wide range of applications ranging from biosensor surfaces to nanoparticles with tremendous potential for use in diagnostic assays for example in the detection of cancer, autoimmune and neurodegenerative related diseases.
Malcolm has authored over 100 peer-reviewed articles and reviews and holds two patents.